Mark Currie is an Assistant Professor in the Department of Biology at the University of Toronto Mississauga. He received BSc and PhD degrees in Biochemistry from Queen’s University and completed postdoctoral research in the Department of Cell Biology at Harvard Medical School. His lab employs structural biology, biochemistry and cellular model systems to answer both fundamental and translational questions related to genome organization and epigenetic gene regulation.
Current Courses
Publications
Wong, S.J., Ringel, A.E., Yuan, W., Paulo, J.A., Yoon, H., Currie, M.A. & Haigis, M.C. (2021). Development of a colorimetric α-ketoglutarate detection assay for prolyl hydroxylase domain (PHD) proteins. J. Biol. Chem. 296.
Iglesias, N*., Currie, M.A.*, Jih, G., Siuti, N., Paulo, J.A., Kalocsay, M., Gygi, S.P., & Moazed, D. (2018) Automethylation-induced conformational switch in Clr4 (Suv39h) maintains epigenetic stability. Nature 560, 504-508.
Jih, G., Iglesias, N., Currie, M.A., Bhanu, N.V., Paulo, J.A., Gygi, S.P., Garcia, B.A., & Moazed, D. (2017) Unique roles for histone H3K9me states in RNAi and heritable silencing of transcription. Nature 547, 463-467.
Currie, M.A., Brown, G., Wong, A., Ohira, T., Sugiyama, K., Suzuki, T. Yakunin, A., & Jia, Z. (2017) Structural and functional characterization of the TYW3/Taw3 class of SAM-dependent methyltransferase. RNA 23, 346-354.
Behrouzi, R.*, Lu, C.*, Currie, M.A., Jih, G., Iglesias, N., & Moazed, D. (2016) Heterochromatin assembly by interrupted Sir3 bridges across neighboring nucleosomes. Elife 5, e17556.
Ye, Q., Feng, Y., Yin, Y., Faucher, F., Currie, M.A., Rahman, M.N., Jin, J., Li, S., Wei, Q., & Jia, Z. (2013) Structural basis of calcineurin activation by calmodulin. Cell. Signal. 12, 2661-2667.
Wang, F.*, Li, G.*, Altaf, M., Lu, C., Currie, M.A., Johnson, A.M., & Moazed, D. (2013) Heterochromatin protein Sir3 induces contacts between the amino terminus of histone H4 and nucleosomal DNA. Proc. Natl. Acad. Sci. USA 110, 8495-8500.
Currie, M.A., Cameron, K., Dias, F.M., Spencer, H.L., Bayer, E.A., Fontes, C.M., Smith, S.P., & Jia, Z. (2013) Small angle X-ray scattering analysis of Clostridium thermocellum cellulosome N-terminal complexes reveals a highly dynamic structure. J. Biol. Chem. 288, 7978-7985.
Currie, M.A., Adams, J.J., Faucher, F., Bayer, E.A., Jia, Z. & Smith, S.P. (2012) Scaffoldin conformation and dynamics revealed by a ternary complex from the Clostridium thermocellum cellulosome. J. Biol. Chem. 287, 26953-26961.
Currie, M.A., Adams, J.J., Bayer, E.A., Smith, S.P., & Jia, Z. (2010) Purification and crystallization of a multimodular heterotrimeric complex containing both type I and type II cohesion dockerin interactions from the cellulosome of Clostridium thermocellum. Acta. Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66, 327-329.
Adams, J.J., Currie, M.A., Ali, S., Bayer, E.A., Jia, Z., and S.P. Smith (2010) Insights into higher order organization of the cellulosome revealed by a dissect-and-build approach: Crystal structure of interacting Clostridium thermocellum multimodular components. J. Mol. Biol. 396, 833-839.
Currie, M.A.*, Merino, F*., Skarina, T*., Wong, A.H., Singer, A., Brown, G., Savchenko, A., Caniuguir, A., Guixé, V., Yakunin, A.F., & Jia, Z. (2009) ADP-dependent 6-phosphofructokinase from Pyrococcus horikoshii OT3: Structure determination and biochemical characterization of PH1645. J. Biol. Chem. 284, 22664-22671.
Matte, A., Kozlov, G., Trempe, J-F., Currie, M.A., Burke, D., Jia, Z., Gehring, K., Ekiel, I., Beguis, A.M., & Cygler, M. (2009) Preparation and Characterization of Bacterial Protein Complexes for Structural Analysis. Chapter in Adv. Protein Chem. Struct. Biol. (Elsevier Inc. Edited by Andrzej Joachimiak) 76, 1-42.